How to Reconstitute PNC-27: A Step-by-Step Guide
Reconstituting PNC-27 is not identical to reconstituting any other compound in this library. An amphipathic, membrane-active peptide that can aggregate at higher concentrations.
Overview
Make a stock and dilute it into your assay rather than working at the very top of its solubility; some protocols use a DMSO stock for cell work.
What PNC-27 actually is
A designed peptide studied only in the lab, in cell culture, for a striking trick: it latches onto HDM-2 on certain cancer cells and pokes holes in their outer membrane, breaking those cells apart while reportedly leaving healthy cells alone.
Supplied for laboratory research use only — not for human or animal use.
Third-party tested by HPLC and LC-MS, ≥99% purity, with a Certificate of Analysis on every order. Ships across Canada.
Technical detail below
Diluent selection for PNC-27
An amphipathic, membrane-active peptide that can aggregate at higher concentrations. Prepare a stock and dilute into the assay system rather than working at the solubility limit; some cell-assay protocols use a DMSO stock.
Common reconstitution reference
A 5 mg vial in 2 mL yields 2.5 mg/mL. Prepare fresh working dilutions for cell assays.
Open the PNC-27 calculatorMethod notes for this compound
- For laboratory in-vitro use only — this compound is studied strictly in cell-culture research.
- Prepare a stock and dilute into the assay system rather than dissolving at high concentration.
- Aliquot and refrigerate; prepare fresh working dilutions.
What PNC-27 is studied for
Studied for binding HDM-2 displayed on the surface of certain cancer cells in vitro.
Examined for forming transmembrane pores that lead to lysis of targeted cells in culture.
Investigated for a reported preference for tumour cells over normal cells in preclinical cell-culture models.
Summarizes published preclinical literature. Provided for research reference only; not a claim of efficacy or a description of human use.
More PNC-27 reference
Lyophilized and reconstituted storage conditions, plus the practical working window.
Which solvents work, why, and what abnormal dissolution behaviour indicates.
The specific chemical routes by which this molecule breaks down, and how to limit each.
Which assays are informative for this molecule, and what to actually check on its COA.
Compound-specific bench practices, and the errors most often made with this molecule.
What to inspect on arrival, and which conditions actually warrant rejecting a vial.
Questions specific to this compound — structure, chemistry, and common misconceptions.
Reconstitution reference for other compounds
PNC-27 is supplied strictly as a research chemical for in-vitro laboratory and research use only. It is not intended for human or animal consumption, diagnostic, or therapeutic use. This page is educational laboratory-handling reference information — not medical advice, not usage guidance, and not a protocol.