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PNC-27 Reconstitution Calculator

For in vitro research use only · Not medical advice

Preset to a 5 mg PNC-27 vial in 2 mL of diluent. A 5 mg vial in 2 mL yields 2.5 mg/mL. Prepare fresh working dilutions for cell assays.

PNC-27 reconstitution

Preset to a common PNC-27 vial size and diluent volume. Adjust any field for your own laboratory protocol.

mg
mL
mcg
Concentration
2.5
mg/mL
Portion volume
0.200
mL
Portions / vial
10
per vial

A 5 mg PNC-27 vial reconstituted with 2 mL gives 2.5 mg/mL. Measuring 0.200 mL gives 500 mcg per portion — about 10 portions from the vial.

Choosing a diluent for PNC-27

An amphipathic, membrane-active peptide that can aggregate at higher concentrations. Prepare a stock and dilute into the assay system rather than working at the solubility limit; some cell-assay protocols use a DMSO stock.

PrimarySterile water for a stock, then dilution into assay buffer
Alternative 1DMSO for concentrated stocks in cell work
Alternative 2Bacteriostatic water for multi-use handling

Handling notes for PNC-27

  • For laboratory in-vitro use only — this compound is studied strictly in cell-culture research.
  • Prepare a stock and dilute into the assay system rather than dissolving at high concentration.
  • Aliquot and refrigerate; prepare fresh working dilutions.

After reconstitution

StorageRefrigerate at 2-8 °C and use promptly; prepare fresh working dilutions for assays.
Working windowShort — prepare working solutions close to use.
Freeze–thawAliquot on first reconstitution; the amphipathic sequence is best not repeatedly frozen and thawed.
Full PNC-27 storage reference
Need bacteriostatic water?

USP-grade sterile bacteriostatic water, 10 mL multi-use vial.

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Research Use Only. This calculator is a convenience reference for laboratory measurement. All values are for in-vitro research applications only. Popular Peptides does not provide medical advice. Products sold by Popular Peptides are not approved by Health Canada for human or veterinary therapeutic use. Always follow qualified research protocols and institutional guidelines.