How to Reconstitute Glutathione: A Step-by-Step Guide
Reconstituting Glutathione is not identical to reconstituting any other compound in this library. Very water-soluble — far more so than most peptides here — and dissolves in seconds.
Overview
It dissolves almost instantly in sterile or bacteriostatic water. A 1500 mg vial in 10 mL gives 150 mg/mL. The liquid will be slightly acidic, which is normal and actually helps it keep.
What Glutathione actually is
Glutathione is a very small peptide — just three building blocks, glutamate, cysteine and glycine — that nearly every cell makes for itself. Its sulfur group grabs reactive oxygen, which makes it the main built-in antioxidant researchers measure when they study oxidative stress. This is the reduced form, called GSH.
Supplied for laboratory research use only — not for human or animal use.
Third-party tested by HPLC and LC-MS, ≥99% purity, with a Certificate of Analysis available on request. Ships across Canada.
Technical detail below
Diluent selection for Glutathione
Very water-soluble — far more so than most peptides here — and dissolves in seconds. A fresh aqueous solution is acidic (around pH 3) because of its two carboxyl groups; that low pH actually slows oxidation of the thiol. Raising the pH toward neutral or alkaline, or trace metal ions such as copper and iron, speeds conversion to the disulfide GSSG.
Common reconstitution reference
A 1500 mg vial in 10 mL gives 150 mg/mL. Glutathione is worked at milligram strengths — the reference aliquot here is 50 mg (50,000 mcg).
Open the Glutathione calculatorMethod notes for this compound
- Let the vial reach room temperature before opening to stop condensation getting in.
- Use degassed diluent where possible and cap promptly after drawing.
- Avoid metal tools and buffers with trace copper or iron; plastic or glass only.
- Label aliquots with reconstitution date and diluent.
What Glutathione is studied for
Glutathione is the most abundant low-molecular-weight thiol in most cells, and the ratio of reduced to oxidised glutathione is widely used as a read-out of oxidative stress in cell-culture and tissue studies.
Meister and Anderson (Annu Rev Biochem 1983) reviewed glutathione synthesis by γ-glutamylcysteine synthetase and glutathione synthetase, and its breakdown by γ-glutamyl transpeptidase — the framework most synthesis and turnover studies still use.
GSTs conjugate glutathione to electrophilic compounds, and this conjugation step is a standard model in xenobiotic-metabolism research.
Glutathione peroxidases use GSH to reduce hydrogen peroxide and lipid hydroperoxides, with glutathione reductase recycling GSSG back to GSH using NADPH — a common model for studying peroxide handling in vitro.
Summarizes published preclinical literature — see the selected references below. Provided for research reference only; not a claim of efficacy or a description of human use.
References describe in-vitro and preclinical research and are listed for reference only — not evidence of efficacy and not a description of human use.
More Glutathione reference
Lyophilized and reconstituted storage conditions, plus the practical working window.
Which solvents work, why, and what abnormal dissolution behaviour indicates.
The specific chemical routes by which this molecule breaks down, and how to limit each.
Which assays are informative for this molecule, and what to actually check on its COA.
Compound-specific bench practices, and the errors most often made with this molecule.
What to inspect on arrival, and which conditions actually warrant rejecting a vial.
Questions specific to this compound — structure, chemistry, and common misconceptions.
Reconstitution reference for other compounds
Reviewed for research-use compliance by Kobi Williams, Founder — Popular Peptides Canada. Last reviewed 22 July 2026.
Glutathione is supplied strictly as a research chemical for in-vitro laboratory and research use only. It is not intended for human or animal consumption, diagnostic, or therapeutic use. This page is educational laboratory-handling reference information — not medical advice, not usage guidance, and not a protocol.