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GHK-Cu Reconstitution Calculator

For in vitro research use only · Not medical advice

Preset to a 100 mg GHK-Cu vial in 5 mL of diluent. A 100 mg vial in 5 mL gives 20 mg/mL. The solution should be a clear, even blue — use neutral or slightly alkaline diluent only.

GHK-Cu reconstitution

Preset to a common GHK-Cu vial size and diluent volume. Adjust any field for your own laboratory protocol.

mg
mL
mcg
Concentration
20
mg/mL
Portion volume
0.050
mL
Portions / vial
100
per vial

A 100 mg GHK-Cu vial reconstituted with 5 mL gives 20 mg/mL. Measuring 0.050 mL gives 1,000 mcg per portion — about 100 portions from the vial.

Choosing a diluent for GHK-Cu

Freely water-soluble, and unmistakable: GHK-Cu solutions are a distinct blue. That colour is the copper(II) coordination itself, which makes this the one compound here whose integrity you can partly assess by eye. A properly reconstituted vial gives a clear, evenly blue solution.

PrimarySterile or bacteriostatic water
Alternative 1Slightly alkaline buffer (pH 7–8) where complex stability is prioritised

Handling notes for GHK-Cu

  • Never reconstitute in acidic diluent — low pH dissociates the copper complex.
  • Keep chelating agents such as EDTA out of any buffer used with this compound.
  • Treat colour change as a discard signal: clear blue is correct, pale or green is not.
  • Avoid contact with reducing agents, which will reduce Cu(II) to Cu(I) and collapse the complex.

After reconstitution

StorageRefrigerate at 2–8 °C, protected from light. Solution pH matters unusually much here: the copper-histidine coordination weakens as pH falls, and the complex can dissociate in acidic conditions.
Working windowCommonly worked with for 2–4 weeks at 2–8 °C.
Freeze–thawAliquot on reconstitution. Freeze–thaw cycling risks local pH shifts during ice formation, which is a specific hazard for a pH-sensitive coordination complex.
Full GHK-Cu storage reference
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Research Use Only. This calculator is a convenience reference for laboratory measurement. All values are for in-vitro research applications only. Popular Peptides does not provide medical advice. Products sold by Popular Peptides are not approved by Health Canada for human or veterinary therapeutic use. Always follow qualified research protocols and institutional guidelines.