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AOD-9604 Reconstitution Calculator

For in vitro research use only · Not medical advice

Preset to a 10 mg AOD-9604 vial in 2 mL of diluent. A 10 mg vial in 2 mL yields 5 mg/mL. Keep the stock near neutral pH and free of reducing agents.

AOD-9604 reconstitution

Preset to a common AOD-9604 vial size and diluent volume. Adjust any field for your own laboratory protocol.

mg
mL
mcg
Concentration
5
mg/mL
Portion volume
0.100
mL
Portions / vial
20
per vial

A 10 mg AOD-9604 vial reconstituted with 2 mL gives 5 mg/mL. Measuring 0.100 mL gives 500 mcg per portion — about 20 portions from the vial.

Choosing a diluent for AOD-9604

Water-soluble at ordinary working strengths. Two arginines give it a small net positive charge at neutral pH, which offsets the stretch of hydrophobic residues (Leu, Ile, Val, Phe). Avoid any diluent or buffer containing reducing agents such as DTT, TCEP or β-mercaptoethanol — they open the disulfide that defines the molecule.

PrimaryBacteriostatic water (0.9% benzyl alcohol)
Alternative 1Sterile water (USP grade)
Alternative 2Phosphate-buffered saline

Handling notes for AOD-9604

  • Let the vial reach room temperature before opening.
  • Add diluent gently down the vial wall and swirl; do not vortex.
  • Keep reducing agents (DTT, TCEP, β-mercaptoethanol) and alkaline buffers away from the stock.
  • Label aliquots with reconstitution date and diluent.

After reconstitution

StorageRefrigerate at 2-8 °C and keep near neutral pH; aliquot for anything beyond short-term use.
Working windowCommonly worked with for 2-3 weeks at 2-8 °C in bacteriostatic water.
Freeze–thawAliquot on first reconstitution and freeze aliquots once; repeated cycles concentrate peptide at the ice interface and favour intermolecular disulfide pairing.
Full AOD-9604 storage reference
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USP-grade sterile bacteriostatic water, 10 mL multi-use vial.

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Research Use Only. This calculator is a convenience reference for laboratory measurement. All values are for in-vitro research applications only. Popular Peptides does not provide medical advice. Products sold by Popular Peptides are not approved by Health Canada for human or veterinary therapeutic use. Always follow qualified research protocols and institutional guidelines.