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Epitalon vs PNC-27

Epitalon and PNC-27 are studied in overlapping research areas, which is why they are frequently compared. This is a neutral side-by-side reference drawn from published preclinical literature and laboratory handling data.

Shared research areas:Cellular Longevity
ClassSynthetic tetrapeptide (Ala-Glu-Asp-Gly)Membranolytic fusion peptide (32 residues): p53-derived domain + membrane-penetrating leader
Molecular weight390.35 g/molNot specified
CAS number307297-39-8Not assigned / not specified
Purity spec≥98%≥99%
Research areasCellular Longevity, Circadian & SleepCellular Longevity
Primary diluentSterile or bacteriostatic waterSterile water for a stock, then dilution into assay buffer
Working windowCommonly worked with for 3–4 weeks at 2–8 °C.Short — prepare working solutions close to use.
Lead degradation routeAspartate-glycine isomerisation — the Asp-Gly motif is among the most isomerisation-prone sequences in peptide chemistry, and Epitalon contains it.Aggregation of the amphipathic chain at the air-liquid interface or high concentration.
Freeze–thawHighly tolerant. A four-residue peptide has essentially no structure to disrupt, making this one of the more freeze-tolerant compounds in the catalogue.Aliquot on first reconstitution; the amphipathic sequence is best not repeatedly frozen and thawed.
Light sensitivityNo specific light requirement beyond normal practice.No specific light requirement beyond normal practice.

How they actually differ

Comparing the two: Epitalon is synthetic tetrapeptide (ala-glu-asp-gly), while PNC-27 is membranolytic fusion peptide (32 residues): p53-derived domain + membrane-penetrating leader — different molecular classes with different handling consequences; they call for different primary diluents (sterile or bacteriostatic water versus sterile water for a stock, then dilution into assay buffer); their leading degradation routes differ (aspartate-glycine isomerisation for Epitalon, aggregation of the amphipathic chain at the air-liquid interface or high concentration. for PNC-27), so the storage precautions that matter are not the same; their practical working windows differ once reconstituted. The sections below set out each in full.

Epitalon — origin

Epitalon is a four-residue peptide derived by Vladimir Khavinson's group from Epithalamin, a pineal gland extract studied in the Soviet Union from the 1970s. It represents the reductionist end of the peptide field — the attempt to identify the shortest sequence retaining the activity of a complex tissue extract. At 390 Da it is the smallest compound in this catalogue by a wide margin.

PNC-27 — origin

PNC-27 is a designed 32-residue peptide fusing a p53-derived region that binds HDM-2 with a membrane-penetrating leader sequence. It is studied strictly in vitro for a reported ability to form pores in the membranes of cancer cells that display HDM-2 at their surface, while reportedly sparing normal cells.

Epitalon research themes

Telomerase activation

The most-cited claim in the Epitalon literature, examined in cell-culture models.

Pineal and melatonin rhythm

Follows from its Epithalamin origin; studied for effects on circadian signalling in animal models.

Ageing models

A long-running Russian research programme examined lifespan endpoints in rodent models.

Peptide bioregulator concept

Epitalon is the flagship of Khavinson's "peptide bioregulator" framework, a distinct research tradition worth understanding as context.

PNC-27 research themes

HDM-2 cell-surface targeting

Studied for binding HDM-2 displayed on the surface of certain cancer cells in vitro.

Membrane pore formation

Examined for forming transmembrane pores that lead to lysis of targeted cells in culture.

Selectivity in vitro

Investigated for a reported preference for tumour cells over normal cells in preclinical cell-culture models.

Epitalon handling

  • Do not over-engineer storage for this compound — refrigeration and a sound seal are genuinely sufficient.
  • Avoid prolonged storage of reconstituted solution, since Asp-Gly isomerisation is slow but cumulative.
  • Verify the analytical method behind any purity figure, as short polar peptides are easy to under-resolve.

PNC-27 handling

  • For laboratory in-vitro use only — this compound is studied strictly in cell-culture research.
  • Prepare a stock and dilute into the assay system rather than dissolving at high concentration.
  • Aliquot and refrigerate; prepare fresh working dilutions.

Both third-party tested

Every Popular Peptides batch of Epitalon and PNC-27 is independently tested by HPLC and LC-MS with a published Certificate of Analysis. Enter a lot number to pull the COA for a specific vial.

Epitalon reference

PNC-27 reference

Related comparisons

Epitalon and PNC-27 are supplied strictly as research chemicals for in-vitro laboratory and research use only. They are not intended for human or animal consumption, diagnostic, or therapeutic use. This comparison summarizes published preclinical literature and laboratory handling data; it is not medical advice, not a claim of efficacy, and not usage guidance.