NAD+ Reconstitution Calculator
For in vitro research use only · Not medical advice
Preset to a 500 mg NAD+ vial in 4 mL of diluent. A 500 mg vial in 4 mL yields 125 mg/mL. Let the vial reach room temperature before opening — NAD+ is strongly hygroscopic.
NAD+ reconstitution
Preset to a common NAD+ vial size and diluent volume. Adjust any field for your own laboratory protocol.
A 500 mg NAD+ vial reconstituted with 4 mL gives 125 mg/mL. Measuring 0.400 mL gives 50,000 mcg per portion — about 10 portions from the vial.
Choosing a diluent for NAD+
Very freely water-soluble — far more so than any peptide here — and the reason vial sizes are measured in hundreds of milligrams rather than single digits. Solutions are clear and faintly yellow at high concentration. Critically, NAD+ is stable in acidic solution and rapidly degraded in alkaline solution, the reverse of the pH preference of many peptides.
Handling notes for NAD+
- Allow the sealed vial to reach room temperature before opening — opening a cold vial of hygroscopic material condenses water directly onto it.
- Keep solutions at or below neutral pH; alkaline conditions destroy NAD+ quickly.
- Prepare fresh solutions where concentration accuracy is important rather than relying on stored stock.
- Protect from light at all stages.
After reconstitution
USP-grade sterile bacteriostatic water, 10 mL multi-use vial.
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Research Use Only. This calculator is a convenience reference for laboratory measurement. All values are for in-vitro research applications only. Popular Peptides does not provide medical advice. Products sold by Popular Peptides are not approved by Health Canada for human or veterinary therapeutic use. Always follow qualified research protocols and institutional guidelines.