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Glutathione Reconstitution Calculator

For in vitro research use only · Not medical advice

Preset to a 1500 mg Glutathione vial in 10 mL of diluent. A 1500 mg vial in 10 mL gives 150 mg/mL. Glutathione is worked at milligram strengths — the reference aliquot here is 50 mg (50,000 mcg).

Glutathione reconstitution

Preset to a common Glutathione vial size and diluent volume. Adjust any field for your own laboratory protocol.

mg
mL
mcg
Concentration
150
mg/mL
Portion volume
0.333
mL
Portions / vial
30
per vial

A 1500 mg Glutathione vial reconstituted with 10 mL gives 150 mg/mL. Measuring 0.333 mL gives 50,000 mcg per portion — about 30 portions from the vial.

Choosing a diluent for Glutathione

Very water-soluble — far more so than most peptides here — and dissolves in seconds. A fresh aqueous solution is acidic (around pH 3) because of its two carboxyl groups; that low pH actually slows oxidation of the thiol. Raising the pH toward neutral or alkaline, or trace metal ions such as copper and iron, speeds conversion to the disulfide GSSG.

PrimarySterile water (USP grade)
Alternative 1Bacteriostatic water (0.9% benzyl alcohol)
Alternative 2Phosphate-buffered saline (degassed)

Handling notes for Glutathione

  • Let the vial reach room temperature before opening to stop condensation getting in.
  • Use degassed diluent where possible and cap promptly after drawing.
  • Avoid metal tools and buffers with trace copper or iron; plastic or glass only.
  • Label aliquots with reconstitution date and diluent.

After reconstitution

StorageRefrigerate at 2-8 °C in a full, tightly capped vial to limit headspace oxygen; freeze aliquots for anything beyond a few days.
Working windowCommonly worked with for about 1 week at 2-8 °C; frozen aliquots keep longer.
Freeze–thawTolerates a few freeze-thaw cycles, but each thaw exposes the thiol to dissolved oxygen. Aliquot once and thaw each portion only once.
Full Glutathione storage reference
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USP-grade sterile bacteriostatic water, 10 mL multi-use vial.

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Research Use Only. This calculator is a convenience reference for laboratory measurement. All values are for in-vitro research applications only. Popular Peptides does not provide medical advice. Products sold by Popular Peptides are not approved by Health Canada for human or veterinary therapeutic use. Always follow qualified research protocols and institutional guidelines.