How to Read a TB-500 (Thymosin Beta-4) Certificate of Analysis (COA)
A Certificate of Analysis is only useful if you know which number on it matters for the compound in front of you. For TB-500 (Thymosin Beta-4), the informative checks are not the same as for a generic short peptide.
In plain English
The most useful thing to look at is the weight measurement, not the purity percentage. Scan just above the expected weight for a small companion peak — that indicates oxygen damage. A report can show excellent purity while that companion peak is present, which tells you the material was fine when made but has aged since.
What TB-500 (Thymosin Beta-4) actually is
TB-500 is a lab-made fragment of Thymosin Beta-4, a protein present in almost every cell in the body and especially concentrated in the fluid around a wound. Research on it centres on cell movement — the internal scaffolding that lets a cell change shape and travel where it is needed.
Supplied for laboratory research use only — not for human or animal use.
Third-party tested by HPLC and LC-MS, ≥99% purity, with a Certificate of Analysis on every order. Ships across Canada.
Technical detail below
Assays that are informative for TB-500 (Thymosin Beta-4)
What to check on the COA
The most informative check for TB-500 is the mass spectrum: look for a +16 Da satellite indicating methionine oxidation. A COA showing high HPLC purity but a visible +16 species describes material that was pure at manufacture and has partly oxidised since.
Oxidised (sulfoxide) species and truncation products from incomplete coupling.
Verify a Popular Peptides batch
Every batch is third-party tested by HPLC and LC-MS with a published Certificate of Analysis (≥99% purity). Enter a lot number to pull the COA for the exact vial in front of you.
What TB-500 (Thymosin Beta-4) is studied for
The defining studied mechanism: binding G-actin and influencing the polymerisation equilibrium that governs cell motility.
Investigated in models where directed cell movement into a tissue defect is the measured endpoint.
Two of the better-populated preclinical literatures for the parent protein.
Studied for effects on inflammatory signalling in tissue-injury models.
Summarizes published preclinical literature. Provided for research reference only; not a claim of efficacy or a description of human use.
More TB-500 (Thymosin Beta-4) reference
Lyophilized and reconstituted storage conditions, plus the practical working window.
Diluent selection, dissolution behaviour, and the calculator preset for this compound.
Which solvents work, why, and what abnormal dissolution behaviour indicates.
The specific chemical routes by which this molecule breaks down, and how to limit each.
Compound-specific bench practices, and the errors most often made with this molecule.
What to inspect on arrival, and which conditions actually warrant rejecting a vial.
Questions specific to this compound — structure, chemistry, and common misconceptions.
Purity & COA reference for other compounds
TB-500 (Thymosin Beta-4) is supplied strictly as a research chemical for in-vitro laboratory and research use only. It is not intended for human or animal consumption, diagnostic, or therapeutic use. This page is educational laboratory-handling reference information — not medical advice, not usage guidance, and not a protocol.