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How to Read a Wolverine (BPC-157 + TB-500) Certificate of Analysis (COA)

A Certificate of Analysis is only useful if you know which number on it matters for the compound in front of you. For Wolverine (BPC-157 + TB-500), the informative checks are not the same as for a generic short peptide.

Two-component repair blend — BPC-157 (5 mg) + TB-500 (5 mg) in one 10 mg vialTissue RegenerationMusculoskeletal

Overview

A blend report should show both parts — two main peaks and both weights (about 1419 and 4963) — and ideally the ratio. Check just above the TB-500 weight for a small +16 damage peak.

What Wolverine (BPC-157 + TB-500) actually is

One vial holding the two most-studied repair peptides together: BPC-157 (5 mg) and TB-500 (5 mg) in one 10 mg vial. BPC-157 is studied around blood-vessel formation and gut and tendon repair; TB-500 around cell movement and wound healing. Two complementary repair routes in one set.

Supplied for laboratory research use only — not for human or animal use.

Research-grade Wolverine (BPC-157 + TB-500)

Third-party tested for HPLC purity and content, ≥99% purity, with a Certificate of Analysis available on request. Ships across Canada.

Technical detail below

Assays that are informative for Wolverine (BPC-157 + TB-500)

Method 1RP-HPLC purity (UV 214 nm)
Method 2LC-MS identity / molecular-ion confirmation
Method 3Appearance and reconstitution clarity
Method 4Water content (Karl Fischer) where specified

What to check on the COA

A blend COA should show both components — two principal peaks on RP-HPLC and both target masses on LC-MS (about 1419.5 Da for BPC-157 and 4963.5 Da for TB-500) — and ideally the ratio between them. Scan just above the TB-500 mass for a +16 Da oxidation companion.

Characteristic impurity profile

Methionine-oxidised TB-500 (+16 Da) and the deletion sequences characteristic of each component.

Verify a Popular Peptides batch

Third-party tested for HPLC purity and content (≥99% purity), with the Certificate of Analysis for your batch available on request. Batch lookups are in our members-only area.

What Wolverine (BPC-157 + TB-500) is studied for

Complementary repair mechanisms

BPC-157 is studied around angiogenesis and growth-factor signalling; TB-500 around actin-mediated cell migration — non-overlapping routes into tissue repair.

Combined tissue-repair models

Studied together in connective-tissue and wound-healing research designs where both mechanisms are of interest.

Why studied as a pair

Because their mechanisms do not overlap, the two are frequently examined in parallel rather than as substitutes for one another.

Summarizes published preclinical literature — see the selected references below. Provided for research reference only; not a claim of efficacy or a description of human use.

References describe in-vitro and preclinical research and are listed for reference only — not evidence of efficacy and not a description of human use.

More Wolverine (BPC-157 + TB-500) reference

Purity & COA reference for other compounds

Wolverine (BPC-157 + TB-500) overview Wolverine (BPC-157 + TB-500) calculatorWolverine (BPC-157 + TB-500) product details

Reviewed for research-use compliance by Kobi Williams, Founder — Popular Peptides Canada. Last reviewed 22 July 2026.

Wolverine (BPC-157 + TB-500) is supplied strictly as a research chemical for in-vitro laboratory and research use only. It is not intended for human or animal consumption, diagnostic, or therapeutic use. This page is educational laboratory-handling reference information — not medical advice, not usage guidance, and not a protocol.