TB-500 (Thymosin Beta-4)
Synthetic Thymosin Beta-4 fragment, lyophilised. For in vitro research use only.
Key facts
- Compound:
- TB-500 (Thymosin Beta-4)
- Purity:
- ≥99%, verified by HPLC
- Identity confirmed by:
- LC-MS, independent third-party lab
- CAS number:
- 77591-33-4
- Molecular weight:
- 4963.5 g/mol
- Certificate of analysis:
- available on request, verifiable by lot number
- Ships from:
- Vancouver, BC — typically 2–5 business days, tracked and insured, no customs
- Intended use:
- in vitro laboratory research only — not for human or veterinary consumption
Page last updated
TB-500 is a synthetic fragment of Thymosin Beta-4.
Buy TB-500 (Thymosin Beta-4) in Canada direct from Popular Peptides. One 10 mg vial, ≥99% purity, CAS 77591-33-4, molecular weight 4963.5 g/mol. Every batch is assayed by HPLC and LC-MS at an independent laboratory, and the batch-specific Certificate of Analysis is available on request. Each vial carries a lot number you can check yourself at popularpeptides.ca/verify before it goes near an experiment. Tracked, insured delivery from Vancouver, BC — typically 2 to 5 business days, with no customs step and no brokerage fee.
Supplied lyophilised for in vitro laboratory research use only; not intended for human or veterinary use.
Primary literature
- Thymosin beta4 activates integrin-linked kinase and promotes cardiac cell migration, survival and cardiac repair — Nature, 2004
Bock-Marquette et al. Established the actin-binding mechanism (ILK/Akt activation) that underpins most later thymosin beta-4 work.
- Thymosin beta4 accelerates wound healing — Journal of Investigative Dermatology, 1999
Malinda et al. Full-thickness rat and mouse wound models; accelerated keratinocyte migration and collagen deposition.
Cited for research context. A citation is not a claim that this compound is safe, effective, or approved for any use.
⚠️ Research Use Only. This compound is intended for in vitro research and laboratory use only. Not for human or veterinary consumption.
TB-500 — compound background
Synthetic fragment of Thymosin Beta-4
TB-500 corresponds to the active region of Thymosin Beta-4, a 43-residue actin-sequestering protein present in virtually every mammalian cell type and abundant in wound fluid and platelets. Research interest followed the observation that the protein's activity in tissue-organisation models is largely retained by a short fragment of it.
Solubility & reconstitution
Highly water-soluble. Thymosin beta family peptides are strongly hydrophilic and intrinsically disordered in free solution, adopting structure only on binding actin — there is no folded core to unfold and no hydrophobic surface to aggregate. Dissolution is rapid and complete in plain aqueous diluent.
Readily worked at 2–5 mg/mL.
Storage
Sealed at -20 °C for long-term storage. The lyophilized cake is hygroscopic and moisture ingress is the primary storage risk. Refrigerate at 2–8 °C. Unlike the disordered short peptides, TB-500 carries a methionine residue, which is the principal oxidation liability and the reason light and headspace air matter more here.
Commonly worked with for 2–4 weeks at 2–8 °C.
Stability
Aliquot after reconstitution. Repeated cycles risk both concentration effects and progressive oxidation from headspace air introduced at each opening.
Reading the Certificate of Analysis
The most informative check for TB-500 is the mass spectrum: look for a +16 Da satellite indicating methionine oxidation. A COA showing high HPLC purity but a visible +16 species describes material that was pure at manufacture and has partly oxidised since.
Background and handling information is educational, for in-vitro laboratory research use only — not medical advice, dosing, or usage guidance, and not a description of use in humans or animals.
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TB-500 laboratory reference
TB-500 (Thymosin Beta-4) is synthetic fragment of thymosin beta-4. Handling requirements follow from that chemistry rather than from generic peptide guidance — the pages below cover each aspect specifically for this compound.
Lyophilized and reconstituted storage conditions, plus the practical working window.
Diluent selection, dissolution behaviour, and the calculator preset for this compound.
Which solvents work, why, and what abnormal dissolution behaviour indicates.
The specific chemical routes by which this molecule breaks down, and how to limit each.
Which assays are informative for this molecule, and what to actually check on its COA.
Compound-specific bench practices, and the errors most often made with this molecule.
What to inspect on arrival, and which conditions actually warrant rejecting a vial.
Questions specific to this compound — structure, chemistry, and common misconceptions.
TB-500 compared with
Reference content is educational laboratory-handling information for in-vitro research use only — not medical advice, not usage guidance, and not a protocol.