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TB-500 (Thymosin Beta-4) Reconstitution Calculator

For in vitro research use only · Not medical advice

Preset to a 10 mg TB-500 (Thymosin Beta-4) vial in 2 mL of diluent. A 10 mg vial in 2 mL yields 5 mg/mL. Aliquot promptly to limit repeated headspace exposure.

TB-500 (Thymosin Beta-4) reconstitution

Preset to a common TB-500 (Thymosin Beta-4) vial size and diluent volume. Adjust any field for your own laboratory protocol.

mg
mL
mcg
Concentration
5
mg/mL
Portion volume
0.500
mL
Portions / vial
4
per vial

A 10 mg TB-500 (Thymosin Beta-4) vial reconstituted with 2 mL gives 5 mg/mL. Measuring 0.500 mL gives 2,500 mcg per portion — about 4 portions from the vial.

Choosing a diluent for TB-500 (Thymosin Beta-4)

Highly water-soluble. Thymosin beta family peptides are strongly hydrophilic and intrinsically disordered in free solution, adopting structure only on binding actin — there is no folded core to unfold and no hydrophobic surface to aggregate. Dissolution is rapid and complete in plain aqueous diluent.

PrimaryBacteriostatic water (0.9% benzyl alcohol)
Alternative 1Sterile water (USP grade)
Alternative 2Phosphate-buffered saline

Handling notes for TB-500 (Thymosin Beta-4)

  • Minimise headspace exposure — each opening introduces oxygen that drives methionine oxidation.
  • Keep reconstituted vials out of direct light, including bench lighting over long sessions.
  • Introduce diluent against the vial wall; the cake is light and can be dispersed by a direct stream before it dissolves.

After reconstitution

StorageRefrigerate at 2–8 °C. Unlike the disordered short peptides, TB-500 carries a methionine residue, which is the principal oxidation liability and the reason light and headspace air matter more here.
Working windowCommonly worked with for 2–4 weeks at 2–8 °C.
Freeze–thawAliquot after reconstitution. Repeated cycles risk both concentration effects and progressive oxidation from headspace air introduced at each opening.
Full TB-500 (Thymosin Beta-4) storage reference
Need bacteriostatic water?

USP-grade sterile bacteriostatic water, 10 mL multi-use vial.

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More TB-500 (Thymosin Beta-4) reference

TB-500 (Thymosin Beta-4) overviewReconstitution guideSolubilityFAQ Verify a batchProduct details

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Research Use Only. This calculator is a convenience reference for laboratory measurement. All values are for in-vitro research applications only. Popular Peptides does not provide medical advice. Products sold by Popular Peptides are not approved by Health Canada for human or veterinary therapeutic use. Always follow qualified research protocols and institutional guidelines.