How Long Does TB-500 (Thymosin Beta-4) Last? Shelf Life & Stability
"Stable" is meaningless without saying stable against what. TB-500 (Thymosin Beta-4) has its own set of degradation routes, and they determine which storage precautions actually matter for it.
In plain English
The clock on this one is oxidation. Its methionine can pick up an oxygen atom, and that happens at the exact spot researchers care about. It shows up on testing as a small extra peak sitting 16 units above the expected weight. Light and trapped air both speed it up.
What TB-500 (Thymosin Beta-4) actually is
TB-500 is a lab-made fragment of Thymosin Beta-4, a protein present in almost every cell in the body and especially concentrated in the fluid around a wound. Research on it centres on cell movement — the internal scaffolding that lets a cell change shape and travel where it is needed.
Supplied for laboratory research use only — not for human or animal use.
Third-party tested by HPLC and LC-MS, ≥99% purity, with a Certificate of Analysis on every order. Ships across Canada.
Technical detail below
Degradation routes specific to TB-500 (Thymosin Beta-4)
- Methionine sulfoxide formation — the dominant chemical degradation route, detectable as an earlier-eluting shoulder on RP-HPLC and a +16 Da species on LC-MS.
- Backbone hydrolysis at acidic pH over extended solution storage.
- Adsorption to glass and plastic surfaces at dilute working concentrations, a general property of highly charged disordered peptides.
Freeze–thaw tolerance
Aliquot after reconstitution. Repeated cycles risk both concentration effects and progressive oxidation from headspace air introduced at each opening.
How storage addresses these routes
Practical window once reconstituted: 2–4 weeks at 2–8 °C. Store reconstituted vials protected from light; methionine oxidation is accelerated by light and dissolved oxygen.
Full TB-500 (Thymosin Beta-4) storage conditionsWhat TB-500 (Thymosin Beta-4) is studied for
The defining studied mechanism: binding G-actin and influencing the polymerisation equilibrium that governs cell motility.
Investigated in models where directed cell movement into a tissue defect is the measured endpoint.
Two of the better-populated preclinical literatures for the parent protein.
Studied for effects on inflammatory signalling in tissue-injury models.
Summarizes published preclinical literature. Provided for research reference only; not a claim of efficacy or a description of human use.
More TB-500 (Thymosin Beta-4) reference
Lyophilized and reconstituted storage conditions, plus the practical working window.
Diluent selection, dissolution behaviour, and the calculator preset for this compound.
Which solvents work, why, and what abnormal dissolution behaviour indicates.
Which assays are informative for this molecule, and what to actually check on its COA.
Compound-specific bench practices, and the errors most often made with this molecule.
What to inspect on arrival, and which conditions actually warrant rejecting a vial.
Questions specific to this compound — structure, chemistry, and common misconceptions.
Stability reference for other compounds
TB-500 (Thymosin Beta-4) is supplied strictly as a research chemical for in-vitro laboratory and research use only. It is not intended for human or animal consumption, diagnostic, or therapeutic use. This page is educational laboratory-handling reference information — not medical advice, not usage guidance, and not a protocol.