How to Read a NAD+ Certificate of Analysis (COA)
A Certificate of Analysis is only useful if you know which number on it matters for the compound in front of you. For NAD+, the informative checks are not the same as for a generic short peptide.
In plain English
Note the different measurement setting — this is read at a different wavelength from peptides, because it is a nucleotide rather than a chain of amino acids. The most telling impurity figure is free nicotinamide, which is the direct breakdown product. Moisture content matters more here than for anything else.
What NAD+ actually is
NAD+ is not a peptide, and that single fact governs everything about handling it. It is a coenzyme — think of it as a rechargeable battery that shuttles energy around inside cells. It is present in every living cell, was first identified in 1906, and almost every peptide habit is wrong for it.
Supplied for laboratory research use only — not for human or animal use.
Third-party tested by HPLC and LC-MS, ≥99% purity, with a Certificate of Analysis on every order. Ships across Canada.
Technical detail below
Assays that are informative for NAD+
What to check on the COA
Note the different detection wavelength: nucleotides are read at 260 nm, not the 214 nm used for peptide bonds. Free nicotinamide content is the most informative impurity figure, since it is the direct hydrolysis product. Water content matters more here than for any peptide, given how hygroscopic the material is.
Free nicotinamide, ADP-ribose, and NADH from partial reduction.
Verify a Popular Peptides batch
Every batch is third-party tested by HPLC and LC-MS with a published Certificate of Analysis (≥99% purity). Enter a lot number to pull the COA for the exact vial in front of you.
What NAD+ is studied for
Sirtuins consume NAD+ as a co-substrate, which links cellular NAD+ availability directly to their activity.
Its canonical role as the central redox carrier of cellular respiration.
PARP enzymes consume NAD+ during DNA damage response, a heavily studied competing demand.
A major driver of current research interest: measured NAD+ levels fall with age across tissues in animal models.
Summarizes published preclinical literature. Provided for research reference only; not a claim of efficacy or a description of human use.
More NAD+ reference
Lyophilized and reconstituted storage conditions, plus the practical working window.
Diluent selection, dissolution behaviour, and the calculator preset for this compound.
Which solvents work, why, and what abnormal dissolution behaviour indicates.
The specific chemical routes by which this molecule breaks down, and how to limit each.
Compound-specific bench practices, and the errors most often made with this molecule.
What to inspect on arrival, and which conditions actually warrant rejecting a vial.
Questions specific to this compound — structure, chemistry, and common misconceptions.
Purity & COA reference for other compounds
NAD+ is supplied strictly as a research chemical for in-vitro laboratory and research use only. It is not intended for human or animal consumption, diagnostic, or therapeutic use. This page is educational laboratory-handling reference information — not medical advice, not usage guidance, and not a protocol.