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Oxytocin Acetate Reconstitution Calculator

For in vitro research use only · Not medical advice

Preset to a 10 mg Oxytocin Acetate vial in 2 mL of diluent. Reconstitute close to the time of use; oxytocin has the shortest practical solution window in this catalogue.

Oxytocin Acetate reconstitution

Preset to a common Oxytocin Acetate vial size and diluent volume. Adjust any field for your own laboratory protocol.

mg
mL
mcg
Concentration
5
mg/mL
Portion volume
0.200
mL
Portions / vial
10
per vial

A 10 mg Oxytocin Acetate vial reconstituted with 2 mL gives 5 mg/mL. Measuring 0.200 mL gives 1,000 mcg per portion — about 10 portions from the vial.

Choosing a diluent for Oxytocin Acetate

Readily water-soluble. The structurally decisive feature is not solubility but the disulfide bridge between Cys1 and Cys6, which closes a six-residue ring and holds the molecule in its active conformation. Everything about oxytocin handling follows from protecting that bond.

PrimarySterile or bacteriostatic water
Alternative 1Acetate-buffered saline
Alternative 2Phosphate-buffered saline (short-term)

Handling notes for Oxytocin Acetate

  • Do not store reconstituted oxytocin at alkaline pH — beta-elimination of the disulfide is irreversible.
  • Avoid vigorous agitation and foaming; interfacial stress drives both aggregation and disulfide scrambling.
  • Keep reducing agents well away from the workflow — any thiol will open the ring.
  • Aliquot on the day of reconstitution rather than repeatedly sampling one vial.

After reconstitution

StorageRefrigerate at 2–8 °C and use promptly. Oxytocin is the least solution-stable compound in this catalogue; its aqueous stability is strongly pH-dependent, with an optimum in the mildly acidic range around pH 4–5.
Working windowShorter than most: commonly worked with within 1–2 weeks at 2–8 °C.
Freeze–thawAliquot immediately. Freeze–thaw cycling is a particular problem here because interfacial stress promotes the dimerisation route.
Full Oxytocin Acetate storage reference
Need bacteriostatic water?

USP-grade sterile bacteriostatic water, 10 mL multi-use vial.

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More Oxytocin Acetate reference

Oxytocin Acetate overviewReconstitution guideSolubilityFAQ Verify a batchProduct details

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Research Use Only. This calculator is a convenience reference for laboratory measurement. All values are for in-vitro research applications only. Popular Peptides does not provide medical advice. Products sold by Popular Peptides are not approved by Health Canada for human or veterinary therapeutic use. Always follow qualified research protocols and institutional guidelines.