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CJC-1295 (No DAC) vs IGF-1 LR3

CJC-1295 (No DAC) and IGF-1 LR3 are studied in overlapping research areas, which is why they are frequently compared. This is a neutral side-by-side reference drawn from published preclinical literature and laboratory handling data.

Shared research areas:Hormonal & EndocrineMetabolic
ClassGHRH(1-29) tetra-substituted analogue (DAC-free)Recombinant 83-residue protein analogue of IGF-1
Molecular weight3367.9 g/molNot specified
CAS numberNot assigned / not specifiedNot assigned / not specified
Purity spec≥99%≥99%
Research areasHormonal & Endocrine, MetabolicHormonal & Endocrine, Metabolic, Musculoskeletal
Primary diluentBacteriostatic water (0.9% benzyl alcohol)Dilute acetic acid (0.1 M) or 10 mM HCl — required for initial dissolution
Working windowCommonly worked with for 2-3 weeks at 2-8 °C.Short: commonly worked with within 1–2 weeks at 2–8 °C, or frozen in single-use aliquots.
Lead degradation routeDeamidation at Asn/Gln residues over long solution storage.Denaturation and aggregation — the dominant failure mode, and one that has no equivalent in short unstructured peptides.
Freeze–thawAliquot on first reconstitution; avoid re-freezing the solution.Single-use aliquots are the standard practice, and here it genuinely matters. IGF-1 LR3 has tertiary structure to lose — unlike the unstructured short peptides in this catalogue, it can denature, and denaturation is not reversible on rewarming.
Light sensitivityNo specific light requirement beyond normal practice.No specific light requirement beyond normal practice.

How they actually differ

Comparing the two: CJC-1295 (No DAC) is ghrh(1-29) tetra-substituted analogue (dac-free), while IGF-1 LR3 is recombinant 83-residue protein analogue of igf-1 — different molecular classes with different handling consequences; they call for different primary diluents (bacteriostatic water (0.9% benzyl alcohol) versus dilute acetic acid (0.1 m) or 10 mm hcl — required for initial dissolution); their leading degradation routes differ (deamidation at asn/gln residues over long solution storage. for CJC-1295 (No DAC), denaturation and aggregation for IGF-1 LR3), so the storage precautions that matter are not the same; their practical working windows differ once reconstituted. The sections below set out each in full.

CJC-1295 (No DAC) — origin

CJC-1295 without DAC is the first 29 residues of growth-hormone-releasing hormone carrying four stabilising substitutions (D-Ala2, Gln8, Ala15, Leu27) that resist DPP-4 cleavage. Omitting the Drug Affinity Complex — the albumin-binding element of the DAC version — gives it a short half-life, which is the property researchers study it for.

IGF-1 LR3 — origin

IGF-1 LR3 is an engineered analogue carrying two changes to native IGF-1: an arginine substitution at position 3 and a 13-residue N-terminal extension. The Arg3 substitution is the functional one — it drastically reduces binding to IGF binding proteins, which normally sequester the great majority of circulating IGF-1. The result is a molecule that stays free rather than bound.

CJC-1295 (No DAC) research themes

Pulsatile GH release

Studied for prompting growth-hormone release in short, physiological pulses via the GHRH receptor.

DPP-4 resistance

The four substitutions are studied for extending the molecule beyond the minutes-long survival of native GHRH.

Complementary pairing

Frequently studied alongside ghrelin-receptor secretagogues such as Ipamorelin, which recruit a second GH-release pathway.

IGF-1 LR3 research themes

IGFBP evasion

The Arg3 substitution reduces binding-protein affinity, which is the entire design rationale.

Cell proliferation

Widely used in cell-culture research as a growth-factor supplement.

Satellite cell activation

Studied in muscle-biology research models.

PI3K/Akt signalling

The canonical downstream pathway examined in IGF-1 receptor research.

CJC-1295 (No DAC) handling

  • Reach room temperature before opening.
  • Swirl to dissolve; do not shake or vortex — foam indicates interfacial stress on the helix.
  • Aliquot and refrigerate; never re-freeze reconstituted solution.

IGF-1 LR3 handling

  • Dissolve in dilute acetic acid or dilute HCl FIRST; do not attempt direct dissolution in water or PBS.
  • Add carrier protein (e.g. 0.1% BSA) for storage of dilute solutions to prevent adsorptive loss.
  • Prepare single-use aliquots — freeze–thaw denaturation is irreversible.
  • Do not vortex; agitation denatures folded proteins at the air–liquid interface.

Both third-party tested

Every Popular Peptides batch of CJC-1295 (No DAC) and IGF-1 LR3 is independently tested by HPLC and LC-MS with a published Certificate of Analysis. Enter a lot number to pull the COA for a specific vial.

CJC-1295 (No DAC) reference

IGF-1 LR3 reference

Related comparisons

CJC-1295 (No DAC) and IGF-1 LR3 are supplied strictly as research chemicals for in-vitro laboratory and research use only. They are not intended for human or animal consumption, diagnostic, or therapeutic use. This comparison summarizes published preclinical literature and laboratory handling data; it is not medical advice, not a claim of efficacy, and not usage guidance.