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Research Comparison

ARA 290 vs TB-500 (Thymosin Beta-4)

ARA 290 and TB-500 (Thymosin Beta-4) are studied in overlapping research areas, which is why they are frequently compared. This is a neutral side-by-side reference drawn from published preclinical literature and laboratory handling data.

Shared research areas:Tissue Regeneration
Class11-amino-acid EPO-derived linear peptideSynthetic fragment of Thymosin Beta-4
Molecular weight1257.4 g/mol4963.5 g/mol
CAS numberNot assigned / not specified77591-33-4
Purity spec≥99%≥99%
Research areasTissue Regeneration, Cognitive & NeurologicalTissue Regeneration, Musculoskeletal, Cardiovascular
Primary diluentBacteriostatic water (0.9% benzyl alcohol)Bacteriostatic water (0.9% benzyl alcohol)
Working windowCommonly worked with for 2-4 weeks at 2-8 °C in bacteriostatic water.Commonly worked with for 2–4 weeks at 2–8 °C.
Lead degradation routeAsparagine/glutamine deamidation is the most plausible slow route for this sequence.Methionine sulfoxide formation — the dominant chemical degradation route, detectable as an earlier-eluting shoulder on RP-HPLC and a +16 Da species on LC-MS.
Freeze–thawAliquot on first reconstitution; the short backbone tolerates a freeze better than folded proteins but repeated cycles still concentrate solutes at the ice interface.Aliquot after reconstitution. Repeated cycles risk both concentration effects and progressive oxidation from headspace air introduced at each opening.
Light sensitivityNo specific light requirement beyond normal practice.Store reconstituted vials protected from light; methionine oxidation is accelerated by light and dissolved oxygen.

How they actually differ

Comparing the two: ARA 290 is 11-amino-acid epo-derived linear peptide, while TB-500 (Thymosin Beta-4) is synthetic fragment of thymosin beta-4 — different molecular classes with different handling consequences; their leading degradation routes differ (asparagine/glutamine deamidation is the most plausible slow route for this sequence. for ARA 290, methionine sulfoxide formation for TB-500 (Thymosin Beta-4)), so the storage precautions that matter are not the same; their practical working windows differ once reconstituted. The sections below set out each in full.

ARA 290 — origin

ARA 290 (Cibinetide) is an 11-residue peptide modelled on the helix-B surface of erythropoietin. It was engineered to engage the innate repair receptor — a heteromer of the EPO receptor and the beta-common receptor — without the erythropoietic (red-blood-cell-stimulating) activity of full-length EPO.

TB-500 (Thymosin Beta-4) — origin

TB-500 corresponds to the active region of Thymosin Beta-4, a 43-residue actin-sequestering protein present in virtually every mammalian cell type and abundant in wound fluid and platelets. Research interest followed the observation that the protein's activity in tissue-organisation models is largely retained by a short fragment of it.

ARA 290 research themes

Innate repair receptor

Studied for selective engagement of the EPOR/beta-common receptor heteromer that mediates tissue-protective signalling, distinct from the classical erythropoietic receptor.

Neuropathic pain models

A substantial share of the published literature examines small-fibre and neuropathic-pain research models.

Anti-inflammatory signalling

Investigated for modulation of inflammatory pathways in preclinical tissue-injury models.

TB-500 (Thymosin Beta-4) research themes

Actin sequestration

The defining studied mechanism: binding G-actin and influencing the polymerisation equilibrium that governs cell motility.

Cell migration models

Investigated in models where directed cell movement into a tissue defect is the measured endpoint.

Cardiac and corneal repair models

Two of the better-populated preclinical literatures for the parent protein.

Inflammation modulation

Studied for effects on inflammatory signalling in tissue-injury models.

ARA 290 handling

  • Reach room temperature before opening the vial.
  • Add diluent gently down the vial wall; do not vortex.
  • Use low-bind labware at low working concentrations to limit adsorption.

TB-500 (Thymosin Beta-4) handling

  • Minimise headspace exposure — each opening introduces oxygen that drives methionine oxidation.
  • Keep reconstituted vials out of direct light, including bench lighting over long sessions.
  • Introduce diluent against the vial wall; the cake is light and can be dispersed by a direct stream before it dissolves.

Both third-party tested

Every Popular Peptides batch of ARA 290 and TB-500 (Thymosin Beta-4) is independently tested by HPLC and LC-MS with a published Certificate of Analysis. Enter a lot number to pull the COA for a specific vial.

Verify a batchPublished COAsHow we test

ARA 290 reference

Full ARA 290 reference ARA 290 storage ARA 290 reconstitution ARA 290 calculatorARA 290 product details

TB-500 (Thymosin Beta-4) reference

Full TB-500 (Thymosin Beta-4) reference TB-500 (Thymosin Beta-4) storage TB-500 (Thymosin Beta-4) reconstitution TB-500 (Thymosin Beta-4) calculatorTB-500 (Thymosin Beta-4) product details

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ARA 290 and TB-500 (Thymosin Beta-4) are supplied strictly as research chemicals for in-vitro laboratory and research use only. They are not intended for human or animal consumption, diagnostic, or therapeutic use. This comparison summarizes published preclinical literature and laboratory handling data; it is not medical advice, not a claim of efficacy, and not usage guidance.