IGF-1 Reconstitution Calculator
For in vitro research use only · Not medical advice
Preset to a 1 mg IGF-1 vial in 1 mL of diluent. A 1 mg vial in 1 mL yields 1 mg/mL. Dissolve first in dilute acetic acid, then dilute; aliquot for single use.
IGF-1 reconstitution
Preset to a common IGF-1 vial size and diluent volume. Adjust any field for your own laboratory protocol.
A 1 mg IGF-1 vial reconstituted with 1 mL gives 1 mg/mL. Measuring 0.100 mL gives 100 mcg per portion — about 10 portions from the vial.
Choosing a diluent for IGF-1
As a folded protein, IGF-1 dissolves poorly at neutral pH and is conventionally taken up first in a mildly acidic solvent, then diluted into the working buffer. A carrier protein is commonly used to limit adsorptive loss. Direct dissolution in plain water or PBS can leave incomplete dissolution — expected chemistry, not a defective vial.
Handling notes for IGF-1
- Dissolve first in dilute acetic acid, then dilute into the working buffer — never expect plain water to take it up fully.
- Use a carrier protein and low-bind labware to prevent adsorptive loss.
- Aliquot for single use; do not re-freeze reconstituted protein.
After reconstitution
USP-grade sterile bacteriostatic water, 10 mL multi-use vial.
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Research Use Only. This calculator is a convenience reference for laboratory measurement. All values are for in-vitro research applications only. Popular Peptides does not provide medical advice. Products sold by Popular Peptides are not approved by Health Canada for human or veterinary therapeutic use. Always follow qualified research protocols and institutional guidelines.